Questions,
answered

No. Bacillus F is a postbiotic: the stable, well-characterised metabolites the Bacillus F organism produces, without the organism. That one fact explains most of how the product behaves. Composition is the same from batch to batch, and nothing depends on bacteria surviving stomach acid and settling in your gut. A probiotic is a living population with a shelf life measured in viable counts. A postbiotic is a finished preparation, so what leaves the fermentation run is what reaches you. The patents and the comet-assay imaging behind it are on the Science page in full, so you can read the evidence rather than take our word for it.

A probiotic delivers live organisms that have to survive stomach acid, reach the gut intact, and then establish themselves. Each step loses some, which is why live-culture products carry cold-chain requirements, expiry-linked viability counts, and a gap between the number on the label and the number that arrives. A postbiotic skips the organism and delivers the finished metabolites, the compounds the bacterium made, directly. Nothing has to stay alive. What is in the applicator on day ninety is what was in it on day one, because the preparation is chemically stable rather than biologically active. That is also why there is no viability count on the label: there is nothing to count.

A sublingual applicator pen. The outer sleeve is white with a liquid-level window so you can see how much is left, and inside sits a clear cartridge with a measured-dose tip. The daily dose is 2 ml, two 1 ml presses, under the tongue in the morning, held a moment and then swallowed. One applicator lasts fifteen days at that dose, so a 30-day protocol is two of them, and that is the unit we sell: $250. No capsules, no powder to weigh, no mixing. We chose the pen because the applicator measures the dose, not you, which keeps thirty days consistent from first to last. Photographs of the pen are on the product page.

Yes. Keep unopened applicators in the freezer. Once you open one, keep it in the fridge between doses. That is the whole instruction: when it arrives, it goes into the freezer first. Nothing in the preparation is alive, so this isn’t about keeping organisms viable; it is simply how the preparation is stored. Keep it out of direct sun and away from heat, and take it out a minute before you use it.

Each applicator carries its batch date and best-before date on the label, so the answer for yours is printed on it. Because the preparation is chemically stable rather than biologically active, it doesn’t drift the way a live-culture product does. There is no viable count declining between manufacture and the label date, and no real difference between one opened early in its window and one opened later. Keep unopened applicators in the freezer and the open one in the fridge, use two as one 30-day protocol, and check the label date if one has been sitting for a while. If a batch date is unclear, write to us with the batch number and we will tell you.

The strain was recovered from Siberian permafrost cores at Mammoth Mountain in Yakutia and revived in culture in 2009. The discovery, the genome sequencing and the patent family (WO2012060729 plus five national patents) are all documented, and the scans are on our Science page rather than summarised. One distinction we keep making because it matters: the age refers to the sediment layer the sample came from, dated by geocryological methods, not to a measurement of the organism itself. So: the permafrost recovery, the culture date, the genome deposit and the patents are documented and readable. What that history means for you is a separate question, and we don’t dress it up.

Single-cell gel electrophoresis. Cells are set in agarose, lysed so only their DNA is left, then placed in an electric field. Intact DNA is too large to move and stays put. Fragmented DNA migrates and forms a visible “comet tail”, and the length and brightness of that tail is the damage in that one cell. Because the readout is per cell rather than an average, it is a standard method for DNA-integrity work, and it is why the reference imaging on the Science page is real comet-assay frames rather than an illustration. We publish the before and after so the method is visible, not described. It is lab work, not a human trial, and it is labelled that way wherever it appears.

No. We don’t claim any, and you should be sceptical of any supplement brand in this category that claims trials without publishing the registration number and the results. Saying this costs us a marketing line. What does exist is on the Science page in full: the recovered and characterised strain, the patent family including WO2012060729, the genome deposit, and laboratory comet-assay work with the imaging attached. That is the whole evidence base, presented as what it is. If human trial data ever exists, it will appear there with its registration number, not here as a sentence.

Two presses of the applicator, 1 ml each, under the tongue in the morning. Hold it there a moment before swallowing; that is the whole sublingual step. Nothing to mix, weigh or time around meals. Two applicators cover a 30-day protocol, and the liquid-level window on the sleeve shows where you are in it. Take it at the same point in the morning each day, not because a missed hour undoes anything, but because a fixed cue is what makes a 90-day window actually run for 90 days. Keep the open applicator in the fridge between doses. If you miss a day, take the next dose as normal rather than doubling it.

Yes. These are three separate mechanisms, not three versions of the same thing: NMN raises NAD+, spermidine induces autophagy, and Bacillus F works on oxidative-stress defence and cellular resilience. Because they don’t overlap, many customers run all three. The head-to-head comparisons in Learn set out what each one is and what documentation stands behind it, including where the evidence is thinner than the marketing. We don’t sell a stack and we don’t claim a synergy, and none of it is medical advice. If you take prescription medication or manage a condition, talk to your doctor before combining anything.

How it feels varies from person to person, so judge it by numbers. A supplement in this category is exactly the sort of thing that mood, sleep and workload will happily take credit for. So take a baseline first, a biological-age panel or an oxidative-stress panel, before you start. Run 90 days. Re-test on the same panel. The protocol is built around that rhythm, which is why one protocol is 30 days and why we suggest three before drawing a conclusion. We make no claim about what your re-test will show. A measurement before and after is the only version of this question that produces an answer.

None are reported for the preparation. But be clear about what that means: we are a wellness supplement, not a medicine, and a clean safety record isn’t a study of interactions with your prescriptions. If you are pregnant, nursing, on prescription medication, or managing a health condition of any kind, talk to your doctor before adding anything to your routine, including this. We would rather you asked and skipped it than took it on our say-so. If your doctor wants to see the evidence first, the patents and the comet-assay imaging are on the Science page.

Anyone under 18, and anyone whose doctor advises against it. Beyond that: if you are pregnant, nursing, on prescription medication or managing a diagnosed condition, ask your doctor first and treat a no as the answer. We don’t sell Bacillus F as a treatment for anything, we make no clinical or disease claims anywhere on this site, and it should never replace medical care or delay it. That isn’t small print. It is the position behind every answer on this page, and the reason the Science page publishes the documents we have instead of claims we don’t.

$250 per 30-day protocol: two 30 ml applicators, thirty daily 2 ml doses. The only other price is the one-year subscription at $199 per protocol. No tiers, no coupons. Shipping is by DHL, free from three applicators and quoted separately below that, confirmed per country in the quote you get when you write to us, so you see the full amount before anything is invoiced. Because we suggest a 90-day window with a baseline and a re-test, most customers budget three protocols rather than one, and we would rather say that here than have it surprise you at the third order. Practitioners, clinics and distributors are priced separately; see the wholesale page.

Worldwide, in monthly batches, with local-language support for the US, UK, Canada, Japan, Germany, France and Spain. Those are the markets where the site, the documents and the replies exist in the local language. Shipping itself isn’t limited to that list. What varies is import rules: supplement classification, permitted ingredients and customs handling differ by country and change without notice, so a published list of destinations would go stale between batches. Instead we confirm your specific destination in the 48-hour reply, with the shipping cost for that country, before anything is invoiced. If your country is a problem, you hear it in the reply, not after paying. Shipping is by DHL: free on three or more applicators; for one or two, quoted separately and added to the total.

Yes. Practitioners, clinics and distributors are handled separately from individual orders, with their own pricing, minimum quantities and documentation supplied directly. Start on the wholesale page, which sets out what we need to know: the market you serve, the volume you expect per month, and whether you dispense to clients or resell. Because fermentation runs are small and released monthly, wholesale allocation is planned a batch ahead, so the first reply is usually as much about timing as price. It runs through info@bacillusf.com, and you get a quote before any invoice.